Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

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406 – 420 of 3,492 results

406

MP Biomedicals™ Tris-Glycine Running Buffer for Native PAGE

Powder buffer salts for making Native-PAGE buffer.

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407

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Thermo Scientific Chemicals MES hydrate, 99+%, for biochemistry

CAS: 1266615-59-1 Molecular Formula: C6H13NO4S Molecular Weight (g/mol): 195.23 MDL Number: MFCD00149409 InChI Key: SXGZJKUKBWWHRA-UHFFFAOYSA-N Synonym: 4-Morpholinoethanesulfonic acid hydrate,MES IUPAC Name: 2-(morpholin-4-yl)ethane-1-sulfonic acid SMILES: OS(=O)(=O)CCN1CCOCC1

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Specifications

CAS	1266615-59-1
Molecular Weight (g/mol)	195.23
MDL Number	MFCD00149409
SMILES	OS(=O)(=O)CCN1CCOCC1
Synonym	4-Morpholinoethanesulfonic acid hydrate,MES
IUPAC Name	2-(morpholin-4-yl)ethane-1-sulfonic acid
InChI Key	SXGZJKUKBWWHRA-UHFFFAOYSA-N
Molecular Formula	C6H13NO4S

408

TBE Sample Buffer, 6X, Thermo Scientific™

Pricing & Availability
Specifications

Color	Blue
Physical Form	Liquid
Chemical Name or Material	Sample Loading Buffer
Concentration	6X

409

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MES, Monohydrate, cGMP Manufactured, 99.5%, Spectrum™ Chemical

CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.25 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N IUPAC Name: 2-(morpholin-4-yl)ethane-1-sulfonic acid hydrate SMILES: O.OS(=O)(=O)CCN1CCOCC1

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Specifications

CAS	145224-94-8
Molecular Weight (g/mol)	213.25
SMILES	O.OS(=O)(=O)CCN1CCOCC1
IUPAC Name	2-(morpholin-4-yl)ethane-1-sulfonic acid hydrate
InChI Key	MIIIXQJBDGSIKL-UHFFFAOYSA-N
Molecular Formula	C6H15NO5S

410

Ethylenediamine Tetraacetic Acid, MP Biomedicals™

CAS: 60-00-4 Molecular Formula: C10H16N2O8 Molecular Weight (g/mol): 292.24 MDL Number: MFCD00003541 InChI Key: KCXVZYZYPLLWCC-UHFFFAOYSA-N Synonym: EDTA, (Ethylenedinitrilo)tetraacetic acid,Edathamil,Ethylenedinitrilotetraacetic acid IUPAC Name: 2-({2-[bis(carboxymethyl)amino]ethyl}(carboxymethyl)amino)acetic acid SMILES: OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

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Specifications

CAS	60-00-4
Molecular Weight (g/mol)	292.24
MDL Number	MFCD00003541
SMILES	OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
Synonym	EDTA, (Ethylenedinitrilo)tetraacetic acid,Edathamil,Ethylenedinitrilotetraacetic acid
IUPAC Name	2-({2-[bis(carboxymethyl)amino]ethyl}(carboxymethyl)amino)acetic acid
InChI Key	KCXVZYZYPLLWCC-UHFFFAOYSA-N
Molecular Formula	C10H16N2O8

411

Thermo Scientific Chemicals PIPES, 1.0M buffer soln., pH 8.0

CAS: 5625-37-6 Molecular Formula: C8H18N2O6S2 Molecular Weight (g/mol): 302.36 MDL Number: MFCD00006159 InChI Key: IHPYMWDTONKSCO-UHFFFAOYSA-N PubChem CID: 79723 ChEBI: CHEBI:44933 IUPAC Name: 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O

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Specifications

PubChem CID	79723
CAS	5625-37-6
Molecular Weight (g/mol)	302.36
ChEBI	CHEBI:44933
MDL Number	MFCD00006159
SMILES	C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
IUPAC Name	2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid
InChI Key	IHPYMWDTONKSCO-UHFFFAOYSA-N
Molecular Formula	C8H18N2O6S2

412

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Thermo Scientific Chemicals BES, 99+%, for biochemistry

CAS: 10191-18-1 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.25 MDL Number: MFCD00007533 InChI Key: AJTVSSFTXWNIRG-UHFFFAOYSA-N Synonym: N, N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid PubChem CID: 73243 ChEBI: CHEBI:39045 SMILES: OCCN(CCO)CCS(O)(=O)=O

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Specifications

PubChem CID	73243
CAS	10191-18-1
Molecular Weight (g/mol)	213.25
ChEBI	CHEBI:39045
MDL Number	MFCD00007533
SMILES	OCCN(CCO)CCS(O)(=O)=O
Synonym	N, N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid
InChI Key	AJTVSSFTXWNIRG-UHFFFAOYSA-N
Molecular Formula	C6H15NO5S

413

MP Biomedicals™ Tricine

Tricine is for use in electrophoresis buffers.

Pricing & Availability

414

Thermo Scientific Chemicals HEPES hemisodium salt

CAS: 103404-87-1 Molecular Formula: C8H17N2NaO4S Molecular Weight (g/mol): 260.28 MDL Number: MFCD00036463 InChI Key: RDZTWEVXRGYCFV-UHFFFAOYSA-M Synonym: 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid sodium salt PubChem CID: 2724248 ChEBI: CHEBI:46758 IUPAC Name: sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonate SMILES: [Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1

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Specifications

PubChem CID	2724248
CAS	103404-87-1
Molecular Weight (g/mol)	260.28
ChEBI	CHEBI:46758
MDL Number	MFCD00036463
SMILES	[Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1
Synonym	4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid sodium salt
IUPAC Name	sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonate
InChI Key	RDZTWEVXRGYCFV-UHFFFAOYSA-M
Molecular Formula	C8H17N2NaO4S

415

Thermo Scientific Chemicals AMPSO, 98+%

CAS: 68399-79-1 Molecular Formula: C7H17NO5S Molecular Weight (g/mol): 227.28 MDL Number: MFCD00041777 InChI Key: ACERFIHBIWMFOR-UHFFFAOYNA-N Synonym: N-(1,1-Dimethyl-2-hydroxyethyl)-3-amino-2-hydroxypropanesulfonic acid PubChem CID: 100211 IUPAC Name: 2-hydroxy-3-[(1-hydroxy-2-methylpropan-2-yl)amino]propane-1-sulfonic acid SMILES: CC(C)(CO)NCC(O)CS(O)(=O)=O

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Specifications

PubChem CID	100211
CAS	68399-79-1
Molecular Weight (g/mol)	227.28
MDL Number	MFCD00041777
SMILES	CC(C)(CO)NCC(O)CS(O)(=O)=O
Synonym	N-(1,1-Dimethyl-2-hydroxyethyl)-3-amino-2-hydroxypropanesulfonic acid
IUPAC Name	2-hydroxy-3-[(1-hydroxy-2-methylpropan-2-yl)amino]propane-1-sulfonic acid
InChI Key	ACERFIHBIWMFOR-UHFFFAOYNA-N
Molecular Formula	C7H17NO5S

416

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Thermo Scientific Chemicals TAPS, 99+%, for biochemistry

CAS: 29915-38-6 Molecular Formula: C7H17NO6S Molecular Weight (g/mol): 243.27 MDL Number: MFCD00007538 InChI Key: YNLCVAQJIKOXER-UHFFFAOYSA-N Synonym: 3-[Tris(hydroxymethyl)methylamino]-1-propanesulfonic acid PubChem CID: 121591 IUPAC Name: 3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]propane-1-sulfonic acid SMILES: OCC(CO)(CO)NCCCS(O)(=O)=O

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Specifications

PubChem CID	121591
CAS	29915-38-6
Molecular Weight (g/mol)	243.27
MDL Number	MFCD00007538
SMILES	OCC(CO)(CO)NCCCS(O)(=O)=O
Synonym	3-[Tris(hydroxymethyl)methylamino]-1-propanesulfonic acid
IUPAC Name	3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]propane-1-sulfonic acid
InChI Key	YNLCVAQJIKOXER-UHFFFAOYSA-N
Molecular Formula	C7H17NO6S

417

MilliporeSigma™ HEPES, Sodium Salt, ULTROL™ Grade, Calbiochem™,

CAS: 75277-39-3 Molecular Formula: C8H17N2NaO4S Molecular Weight (g/mol): 260.28 MDL Number: MFCD00036463 InChI Key: RDZTWEVXRGYCFV-UHFFFAOYSA-M Synonym: N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic Acid, Na PubChem CID: 2724248 ChEBI: CHEBI:46758 IUPAC Name: sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonate SMILES: [Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1

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Specifications

PubChem CID	2724248
CAS	75277-39-3
Molecular Weight (g/mol)	260.28
ChEBI	CHEBI:46758
MDL Number	MFCD00036463
SMILES	[Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1
Synonym	N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic Acid, Na
IUPAC Name	sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonate
InChI Key	RDZTWEVXRGYCFV-UHFFFAOYSA-M
Molecular Formula	C8H17N2NaO4S

418

Gibco™ CTS™ DPBS, calcium, magnesium, bag format

Gibco™ Cell Therapy Systems (CTS) DPBS is the classical Dulbecco's phosphate buffered saline, which is a balanced salt solution used for a variety of cell culture applications.

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419

BES, MP Biomedicals™

CAS: 10191-18-1 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.25 MDL Number: MFCD00007533 InChI Key: AJTVSSFTXWNIRG-UHFFFAOYSA-N Synonym: N,N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid, N,N-Bis(2-hydroxyethyl)taurine PubChem CID: 73243 ChEBI: CHEBI:39045 IUPAC Name: 2-[bis(2-hydroxyethyl)amino]ethane-1-sulfonic acid SMILES: OCCN(CCO)CCS(O)(=O)=O

Pricing & Availability
Specifications

PubChem CID	73243
CAS	10191-18-1
Molecular Weight (g/mol)	213.25
ChEBI	CHEBI:39045
MDL Number	MFCD00007533
SMILES	OCCN(CCO)CCS(O)(=O)=O
Synonym	N,N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid, N,N-Bis(2-hydroxyethyl)taurine
IUPAC Name	2-[bis(2-hydroxyethyl)amino]ethane-1-sulfonic acid
InChI Key	AJTVSSFTXWNIRG-UHFFFAOYSA-N
Molecular Formula	C6H15NO5S

420

Tris Hydrochloride, MP Biomedicals™

Molecular Formula: C4H12ClNO3 Molecular Weight (g/mol): 157.594 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N Synonym: Trometamol, Tromethamine, Tris-[hydroxymethyl]aminomethane, 2-Amino-2-(hydroxymethyl)-1,3-propanediol PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl

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Specifications

PubChem CID	93573
Molecular Weight (g/mol)	157.594
SMILES	C(C(CO)(CO)N)O.Cl
Synonym	Trometamol, Tromethamine, Tris-[hydroxymethyl]aminomethane, 2-Amino-2-(hydroxymethyl)-1,3-propanediol
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride
InChI Key	QKNYBSVHEMOAJP-UHFFFAOYSA-N
Molecular Formula	C4H12ClNO3

Life Science Buffers

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